Transcriptomic insight into the effect of melatonin in Saccharomyces cerevisiae in the presence and absence of oxidative stress
Mercè Sunyer-Figueres, Jenny Vázquez, Albert Mas, M. Jesús Torija, Gemma Beltran
Antioxidants 2020, 9, 947. https://www.mdpi.com/2076-3921/9/10/947
Melatonin plays important roles in biological processes in humans, so its study has growing interest. Saccharomyces cerevisiae produces melatonin during alcoholic fermentation, and recent studies report that it has an antioxidant role in yeast cells, not only directly neutralizing the effects of oxidative stress, also modulating the expression of genes involved in antioxidant response.
In order to study the effects of melatonin in the global gene response of yeast, we determined the transcriptomic state of cells that were supplemented with melatonin and exposed or non-exposed to oxidative stress.
We found that melatonin crossed cellular membranes and regulated the transcription of several genes, related to transport, signaling, antioxidant activity or several metabolisms. The most regulated genes by melatonin were the mitochondrial ones (mainly those related to electron transport chain), and its expression was downregulated in absence of oxidative stress and upregulated in its presence. Therefore, we concluded that melatonin can reprogram different cellular processes to achieve tolerance to oxidative stress.
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